increased cd11b and hypoxia-inducible factors-1alpha expressions in the lung tissue and surfactant protein-d levels in serum are related with acute lung injury in severe malaria of c57bl/6 mice

background: we aimed to reveal the role of cd11b and hypoxia-inducible factors-1alpha (hif-1α) expressions on monocytes and alveolar macrophages of lung tissue, and the levels of serum surfactant protein-d (sp-d) in severe malaria-associated acute lung injury (ali). methods: the c57bl/6 mice were divided into control group, renal malaria group (inoculated with 10 6 plasmodium berghei anka), and cerebral malaria group (inoculated with 10 7 p. berghei anka). the expressions of cd11b and hif-1α in lung tissue were observed by immunohistochemistry, and serum sp-d levels were measured by elisa. this study was conducted from june 2014 to february 2015 in the laboratory of parasitology, faculty of medicine, universitas brawijaya, malang. results: the cd11b expression on pulmonary tissue of renal and cerebral malaria mice were significantly higher than control mice ( p =0.002; p =0.002), as well as the hif-1α expression on pulmonary tissue ( p =0.002; p =0.002). the level of serum sp-d in renal malaria and cerebral malaria mice were significantly higher than control mice ( p =0.002; p =0.002). we found a strong correlation between the expression of cd11b and hif-1α in lung tissue (r=0.937, p =0.000), as well as between cd11b expression and serum sp-d levels (r=0.907, p =0.000) and between hif-1α expression and serum sp-d levels (r=0.913, p =0.000). conclusion: severe malaria-associated ali increased the expression of cd11b and hif-1α in the lung tissue and increased serum sp-d levels of c57bl/6 mice significantly.